Promotionsvortrag Physik: Real-Time Detection of Specific Protein Secretion from Cells During Apoptosis Using a Label-Free Optical Microchip Biosensor

Dez 13
13. Dezember 2017 10:30 Uhr bis 12:00 Uhr
Raum SR A 1.500, Max - Planck - Institut für die Physik des Lichts, Staudtstraße 2, Erlangen

Einladung zum Promotionsvortrag von Herrn Ying – Jen Chen:
„Real-Time Detection of Specific Protein Secretion from Cells During Apoptosis Using a Label-Free Optical Microchip Biosensor“

Applying resonance-based optical platforms to detect particular biomolecules non-invasively has recently attracted considerable attention in scientific circles due to the platforms’ ultra-sensitivity. In this presentation, I will present a platform based on whispering gallery mode biosensors (WGMBs) to achieve non-invasive, label-free, ultra-sensitive and real-time quantitative detection of the release of specific biomarkers from cells during cell death. The WGM is excited via a tapered fiber or a prism, and the concentration of the specific biomarkers in the complex media could be quantitatively determined by tracking the frequency shift of the resonance. Furthermore, a micro-fluidic device is integrated into the WGMB to enable the stable, long-term measurement of cell response by monitoring the release of specific biomarkers to the surrounding medium in real time. To achieve specific sensing, we developed a surface modification approach to immobilize antibodies on the sensor surface and reduce non-specific binding. The sensing results were validated via conventional biochemistry methods, such as Western Blot (WB), MTT and MTS assays. The platform was subsequently used to perform cell cytotoxicity tests. Both neuroglioma H4 cells and their alpha-synuclein protein (aSyn) overexpressed variant WTS H4 cells were treated with rotenone, a compound presumed to play a role in Parkinson’s disease (PD). (Vortrag auf Englisch)

Dem Vortrag schließt sich eine Diskussion von 15 Minuten an. Vortrag und Diskussion sind öffentlich. Diesen Verfahrensteilen folgt ein nicht öffentliches Rigorosum von 45 Minuten