Einladung zum Promotionsvortrag von Frau Janina Lange:
„Microconstrictions – Quantification of cell mechanical properties with a sensitive and high-throughput microfluidic device“
During the past years, it has become more and more evident that not only chemical cell-cell-signaling, but also cell mechanics play a crucial role in many physiological processes. Besides many others, cell stiffness and viscosity were found to change during cell differentiation, apoptosis, and, interestingly, during the progression of diseases. Thus, cell mechanics can be used as a macroscopic read-out to investigate cell-internal processes, which would otherwise be unobservable. In this talk, a microfluidic device for the high-throughput quantification of cell mechanical properties, like cell stiffness and viscosity, is presented. Thereby, hundreds of cells per minute are flushed through an array of micron-scaled constrictions where they are deformed. The read-out of measurement parameters from high-speed movies is demonstrated, so that strains and stresses working on the cells during the deformation can be calculated. Cell deformation can be quantified through power-law theory, which describes the deformation of a visco-elastic body. The device is demonstrated to sensitively measure mechanical changes of the cell nucleus and the cell cytoskeleton. Moreover, the presented measurement device is equipped with a fluorescence extension. Cell mechanical properties can now be correlated with the expression of fluorescently labeled proteins in every investigated cell on a high-throughput basis. (Vortrag auf Englisch)
Dem Vortrag schließt sich eine Diskussion von 15 Minuten an. Vortrag und Diskussion sind öffentlich. Diesen Verfahrensteilen folgt ein nicht öffentliches Rigorosum von 45 Minuten.